D Y Patil AIET M.Tech. 2012: Biochemistry & Biotechnology Syllabus

All India Entrance Test (AIET) 2012 is conducted by Department of Biotechnology and Bioinformatics, Padmashree Dr. D. Y. Patil University for students seeking admission to B.Tech. Biotechnology, B.Tech. Bioinformatics, B.Tech. Biomedical Engineering, M.Tech. Integrated (Biotechnology), M.Tech. Biotechnology, M.Tech. Bioinformatics, M.Tech. Pharmacoinformatics, M.Sc. Genetic Engineering, M.Sc. Biotechnology, M.Sc. Bioinformatics and M.Sc. Environmental Biotechnology. 

Biochemistry Syllabus for D Y Patil AIET M.Tech. 2012

To crack D Y Patil AIET M.Tech. 2012, you should cover following topics of Biochemistry:

• Organization of life. Importance of water. Cell structure and organelles. Structure and function of biomolecules
• Carbohydrates, Lipids, Proteins  and Nucleic acids.  Biochemical separation techniques. Spectroscopic methods;
• UV-visible  and  fluorescence.  Protein  structure,  folding  and  function:  Myoglobin,  Hemoglobin,  Lysozyme, ribonuclease A, Carboxypeptidase and Chymotrypsin. Enzyme kinetics and regulation, Coenzymes.
• Metabolism and bioenergitics. Generation and utilization of ATP. Photosynthesis. Major metabolic pathways and their  regulation.  Biological  membranes.  Transport  across  membranes.  Signal  transduction;  hormones  and neurotransmitters.
• DNA  replication, transcription  and  translation. Biochemical  regulation  of  gene  expression.  Recombinant DNA technology and applications. Genomics and Proteomics.
• The  immune  system.  Active  and  passive  immunity.  Compliment  system.  Antobody  structure  function  and diversity. Cells of the immune system: T, B and macrophages. T and B cell activation. 
• Major histocompatibilty complex. T cell receptor: Immunology techniques: Immunodiffusion immunoelectrophoresis, RIA and ELISA.

Biotechnology Syllabus for D Y Patil AIET M.Tech. 2012

To crack D Y Patil AIET M.Tech. 2012, you should cover following topics of Biotechnology:

• Recombinant DNA technology, Architecture of plant genome; plant tissue culture, methods of gene transfer into plant cells;  manipulation  of phenotypic traits in plants; production of secondary metabolites using suspension and  immobilized  cell  culture;  methods  of  plant  micropropagation;  crop  improvement  and  development  of transgenic  plants.  Animal  cell  metabolism  and  regulation;  nutritional  requirements  for  animal  cell  culture; production of  vaccines;  growth  hormones  using  animal  cell  culture,  hybridoma technology,  vectors  for gene transfer and expression and regulation of gene.

Food Biotechnology

• Scope  of  biotechnology in  food  &  beverage industry  , elementary  ideas of canning  &  packing, sterilization & pasteurization of food products.
• Factors affecting spoilage, methods of food prservation, quality control of food, detecting systems- ELISA, RIA, monoclonal antibodies & DNA probes.
• Dairy products- General points, cheese, yoghurt, butter, cultured buttermilk, cultured sour milk, novel products.
• Cereal products- Bread & baked goods, starch hydrolytes.

Plant Biotechnology

• Historical aspects of plants cell, tissue and organ culture, PTC lab, aseptic conditions, nutritional components of tissue culture medium.
• Initiation & maintenance of callus, organogenesis, plant propagation through tissue culture micropropagations of flowering plants, commercial applications. Somatic embryogenesis, synthetic seeds. Plant Cell cultures as a system  for  production  of  fine  chemicals,  plant  suspension  cultures,  enhancement  of  product  formation, biotransformations, hairy root cultures.

Animal Biotechnology

• Animal Cell  Cultures,  advantages  &  limitations,  major  differences  in vitro, types  of  tissue  culture,  Biology of cultured  cells,  environment,  cells  proliferation,  kinetics,  differentiation,  energy  metabolism,  initiation  & maintenance of culture, development of continuous cell line.
• Applications of animal cell cultures. Production of vaccines, growth hormones, hybidoma technology.

Basic Techniques in Recombinant DNA Technology

• Restriction enzymes, cloning vectors, construction of cDNA & genomic DNA library, cloning strategies, growth & selection of transformed cells. Analysis of gene & gene transcripts, colony hybridization, Southern hybridization, DNA sequencing , PCR.
• Application  of  DNA  technology  in  diagnosis,  of  diseases  gene  therapy,  genetically  engineered  bacteria  in agriculture  &  therapeutics.  Transgenic  animals  -  fish,  mice,  sheep,  methodology  of  transgenesis.  Ethical  & regulatory issues
• Transgenic plants - Artificial & natural methods of gene transfer, transgenic plants, edible vaccines.

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